题目: Highly Sensitive and Selective DNA-Based Detection of Mercury(II) with α-Hemolysin Nanopore
作者:Shuang Wen,†,‡ Tao Zeng,§,‡ Lei Liu,† Kai Zhao,† Yuliang Zhao,*,†,§ Xianjin Liu,|| and Hai-Chen Wu*,†
单位:
†Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China
§National Center for Nanoscience and Technology of China, Beijing 100190, China
||Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Key Lab of Agro-Food Safety and Quality, Ministry of Agriculture, Institute of Food Safety, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China
摘要:
The duplex formation mediated by Hg2+ in a properly designed ssDNA generates a stable hairpin structure, which greatly alters the translocation profile of the ssDNA through α-hemolysin nanopore. From the 2D-events contour plot, the presence of Hg2+ can be confirmed in as little as 30 min at ∼7 nM or higher. The sensor is highly selective to Hg2+, without interference from other metal ions. It can be fabricated from readily available materials, without the processes of synthesis, purification, probe-making, and so forth. This sensing strategy opens new possibilities for detecting many types of analytes which have specific interactions with DNA molecules.
文章发表信息:J. Am. Chem. Soc., 2011, 133 (45), pp 18312–18317
文章网址链接:http://pubs.acs.org/doi/abs/10.1021/ja206983z